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!0% Ammonium persulfate problems

!0% Ammonium persulfate problems - Protein Science

!0% Ammonium persulfate problems - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 07-18-2008, 01:51 PM
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Default !0% Ammonium persulfate problems



I've tried dissolving 0.1g APS in 1ml of distilled/autoclaved water. I then made 100ul aliquots. The solution in the vials will not freeze at -20C for a week and 50% of the gels will not polymerize. I've tried two different bottles of APS. On the half that polymerize, no banding occurs after staining. Does anyone have any suggestions?
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