Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science
Register Search Today's Posts Mark Forums Read

Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


Resource: Mass Spectrometry-based Methods of Proteome Analysis

Resource: Mass Spectrometry-based Methods of Proteome Analysis - Protein Science

Resource: Mass Spectrometry-based Methods of Proteome Analysis - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-02-2008, 08:03 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Post Resource: Mass Spectrometry-based Methods of Proteome Analysis



Mass Spectrometry-based Methods of Proteome Analysis --> [Only registered users see links. ]

Boris L. Zybailov and Michael P. Washburn
Stowers Institute for Medical Research, Kansas City, MO, USA

1 Principles and Instrumentation 3
1.1 Proteome and Proteomics 3
1.2 Need for Large-scale Analyses of Gene Products at the Protein Level 3
1.3 General Problems in Proteome Analyses 4
1.4 MS Instrumentation 5
1.4.1 Ionization Methods 6
1.4.2 Mass Analyzers 8
1.4.3 MS/MS 9
1.5 Methods of Sample Fractionation 10
1.5.1 2D-electrophoretic Separation of Complex Protein Mixtures 11
1.5.2 Liquid-phase Separation Methods 12
1.5.3 Affinity Methods (Epitope Tagging) 13
1.6 Protein Identification by MS 14
2 Quantitative Methods of Proteome Analysis Using MS 18
2.1 Metabolic Labeling 18
2.2 Isotope Coded Affinity Tags 20
2.3 18O Labeling 21
2.4 Postdigestion Labeling 22
2.5 Global mRNA and Protein Expression Analyses 23
3 Specific Examples of Applications 23
3.1 Global Proteome Sampling 23
3.1.1 Global Proteome Sampling Based on 2D Page 23
3.1.2 Global Proteome Sampling Based on Multidimensional LC 24
3.1.3 MS-assisted Disease Diagnosis from Serum Samples 25
3.2 Analysis of Protein Modifications by Mass Spectrometry 25
3.2.1 Phosphorylated Proteins 27
3.2.2 Glycosylated Proteins 28
3.2.3 Ubiquitinated Proteins 29
3.3 Analysis of Protein–Protein Interactions by Mass Spectrometry 30
3.3.1 Computational Methods of Protein–Protein Interaction Prediction 30
3.3.2 Yeast 2-hybrid Arrays 30
3.3.3 Direct Analysis of Large Protein Complexes; Composition of the
Yeast Ribosome 31
3.3.4 Analysis of Multisubunit Protein Complexes
Involved in Ubiquitin-dependent Proteolysis by Mass Spectrometry 31
3.3.5 Proteomics of the Nuclear Pore Complex 31
3.3.6 High-throughput Analyses of Protein–Protein Interactions 33
3.3.7 Quantitative Proteomics Methods in the Studies of Protein Complexes 35
Reply With Quote
The Following 2 Users Say Thank You to danfive For This Useful Post:
jgure (11-29-2012), lja (12-12-2008)
Reply

Tags
analysis , esi , maldi , mass , mass spectrometry , methods , proteome , resource , spectrometrybased


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
What is Gravity? sdr@sdrodrian.com Physics Forum 1 01-27-2008 02:53 PM
The Achilles Heel of String Theory. S D Rodrian Physics Forum 7 07-08-2006 02:40 PM
GNU units and units.dat; Units of Measurement and Unit Conversion James Redford Physics Forum 0 07-31-2005 12:08 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 10:53 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.19738 seconds with 16 queries