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Bradford Assay Question

Bradford Assay Question - Protein Science

Bradford Assay Question - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 05-15-2008, 05:56 PM
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Post Bradford Assay Question



Hi everyone,

I will need to measure the total protein concentration in my samples.


If i am using bovine serum albumin (BSA) as the protein standard is it necessary to prepare the protein standard dilution using the lysis buffer that I used for my protein extraction?

Now i am using 0.1M sodium phosphate buffer (pH 6.8) and 1% Triton X-100 as my lysis buffers.

Does anybody have protocol for bradford assay where the O.D. values are measured by an ELISA plate reader and not spectrophotometer?

thanks
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