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· · · Protein Pictures 16 photos 9 comments |
· · · Protein Pictures 16 photos 9 comments |
· · · Protein Pictures 16 photos 9 comments | |||
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05-15-2008, 05:56 PM
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 Bradford Assay Question Hi everyone, I will need to measure the total protein concentration in my samples. If i am using bovine serum albumin (BSA) as the protein standard is it necessary to prepare the protein standard dilution using the lysis buffer that I used for my protein extraction? Now i am using 0.1M sodium phosphate buffer (pH 6.8) and 1% Triton X-100 as my lysis buffers. Does anybody have protocol for bradford assay where the O.D. values are measured by an ELISA plate reader and not spectrophotometer? thanks         |
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