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Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

Protein Science
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Caspase-3 Cleavage Assay

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.



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Old 04-15-2008, 10:51 AM
Pipette Filler
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Question Caspase-3 Cleavage Assay

Hello dudes
i am going to assay for caspase enzyme activity.

I have to do the protein cleavage in vitro using a cellular extract in order to analyze the fragments after by immunoprecipitation or westen blot.

The problem is most of the kits i found tested inhibitors' activity or caspase activity by screening and they are not very compatible for using cell extracts and western blots...

Does anyone have a good protocol or assay on how to test for caspases?


any suggestions are appreciated and I wish everyone a nice day
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