| | Re: Sequencing protein after SDS-PAGE
Thanks all! I am not set up for 2-D, but I do have an antibody to my protein of interest so I will try to get it as pure as possible with a protein A column to minimize other proteins at the same (95kD) molecular weight.
Quick question, if I bind IgG to a protein A column, then run my lysate through to retain my p95 target, is it possible to elute just my target protein without the antibody? Or will I need to elute both and then separate them? I am, of course, going to boil in SDS before PAGE, so I guess this would separate the mAb-p95 complexes, correct?