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· · · Protein Pictures 16 photos 9 comments |
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· · · Protein Pictures 16 photos 9 comments | |||
Caspase-3 Cleavage Assay
Protein Science
Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.
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04-15-2008, 10:51 AM
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 Caspase-3 Cleavage Assay Hello dudes  i am going to assay for caspase enzyme activity. I have to do the protein cleavage in vitro using a cellular extract in order to analyze the fragments after by immunoprecipitation or westen blot. The problem is most of the kits i found tested inhibitors' activity or caspase activity by screening and they are not very compatible for using cell extracts and western blots... Does anyone have a good protocol or assay on how to test for caspases? any suggestions are appreciated and I wish everyone a nice day         |
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