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Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

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Protein Extraction Problems

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.



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Old 04-16-2008, 08:57 AM
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Question Protein Extraction Problems

Hello everyone i have a problem in protein extraction using the RIPA lysis buffers.

I added around 1 ml of RIPA lysis buffer to each 6cm dish for the extraction of the protein.

The problem is the sample become very viscous!

I dont know what to do....

can someone please help?

thanks
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