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Lyophilization Denatures Proteins?

Lyophilization Denatures Proteins? - Protein Science

Lyophilization Denatures Proteins? - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 04-13-2008, 09:07 AM
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Question Lyophilization Denatures Proteins?



Hello everyone again,
does lyophilization cause proteins to denature?

I would like to use this method to concentrate my proteins but does this cause me to lose protein function or enzyme activity?

thanks!
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Old 12-16-2009, 07:24 PM
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Default Re: Lyophilization Denatures Proteins?

Quote:
Originally Posted by dnamolecule View Post
Hello everyone again,
does lyophilization cause proteins to denature?

I would like to use this method to concentrate my proteins but does this cause me to lose protein function or enzyme activity?

thanks!
yes. if you use a good stabilizer and good lyophilization cycle your protein dose not denature.
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Old 06-10-2010, 05:26 PM
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Default Re: Lyophilization Denatures Proteins?

I attempted to lyophilize an HRP conjugated Antibody, using the same cycle parameters as for dylight conjugates and unconjugated antibodies. This resulted in significantly reduced activity of the HRP conjugates. Does anyone have an idea what excipients would be helpful to stabilize this protein and enzyme?

Thank you!
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