Gel Electrophoresis and Smeared Bands

oBWhat · #1 (**permalink**) 08-23-2006, 05:00 PM

I just ran a SDS-Gel Electrophoresis and I have smeared bands...

is this due to high voltage / temperature / or just band gel composition or sample buffer?

thanks in advance

kmunson779 · #2 (**permalink**) 08-24-2006, 03:30 PM

One place to look for your answer would be the SDS-PAGE Hall of Shame. This site has pictures of bad SDS-PAGE gels and explains what the problems were. You can look for a gel that looks like yours for your answer.

Otherwise, it's hard to troubleshoot your gel without being able to see it. If you post the image, maybe someone will have some ideas.

oBWhat · #3 (**permalink**) 08-24-2006, 06:10 PM

Wow,
great find kmunson! that helped a lot...thanks

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Member Login

Gel Electrophoresis and Smeared Bands

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.