Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science
Register Search Today's Posts Mark Forums Read

Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


Gel Electrophoresis and Smeared Bands

Gel Electrophoresis and Smeared Bands - Protein Science

Gel Electrophoresis and Smeared Bands - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 08-23-2006, 05:00 PM
oBWhat's Avatar
Graduate Student
Points: 3,826, Level: 41 Points: 3,826, Level: 41 Points: 3,826, Level: 41
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2006
Posts: 124
Thanks: 0
Thanked 7 Times in 7 Posts
Default Gel Electrophoresis and Smeared Bands



I just ran a SDS-Gel Electrophoresis and I have smeared bands...

is this due to high voltage / temperature / or just band gel composition or sample buffer?

thanks in advance
Reply With Quote
  #2  
Old 08-24-2006, 03:30 PM
Graduate Student
Points: 2,923, Level: 35 Points: 2,923, Level: 35 Points: 2,923, Level: 35
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2006
Location: Ithaca, NY
Posts: 145
Thanks: 0
Thanked 17 Times in 12 Posts
Default Re: Gel Electrophoresis and Smeared Bands

One place to look for your answer would be the [Only registered users see links. ]. This site has pictures of bad SDS-PAGE gels and explains what the problems were. You can look for a gel that looks like yours for your answer.

Otherwise, it's hard to troubleshoot your gel without being able to see it. If you post the image, maybe someone will have some ideas.
Reply With Quote
The Following User Says Thank You to kmunson779 For This Useful Post:
jiajia1987 (03-04-2009)
  #3  
Old 08-24-2006, 06:10 PM
oBWhat's Avatar
Graduate Student
Points: 3,826, Level: 41 Points: 3,826, Level: 41 Points: 3,826, Level: 41
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2006
Posts: 124
Thanks: 0
Thanked 7 Times in 7 Posts
Default Re: Gel Electrophoresis and Smeared Bands

Wow,
great find kmunson! that helped a lot...thanks
Reply With Quote
  #4  
Old 02-03-2009, 06:15 PM
Pipette Filler
Points: 2, Level: 1 Points: 2, Level: 1 Points: 2, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Gel Electrophoresis and Smeared Bands

This site was awesome! I totally figured out my disasterous problem too!!
Reply With Quote
Reply

Tags
bands , electrophoresis , gel , smeared


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 09:00 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12374 seconds with 15 queries