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Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

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Isolation of ribosomes with nascent protein

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.



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Old 04-02-2008, 05:30 PM
Pipette Filler
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Talking Isolation of ribosomes with nascent protein

Hallo I want to quintify translation rate of protein of my interest, since i have monoclonal antibodies iwould like to isolate ribosomes with binded nascent protein and this use as antigene in elisa or immunoflorescence. I suggest in vitro translation with mRNA can work but I favour isolation ribosomes from native sample
Can anyone advice me
thanks
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