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Protein Purification Problem

Protein Purification Problem - Protein Science

Protein Purification Problem - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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  #1  
Old 12-29-2007, 12:45 AM
Pipette Filler
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Default Protein Purification Problem



Hello,
I have his-tagged my protein however I am having problems getting it off my column.

Any ideas what is going on?

cheers
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Old 01-03-2008, 05:23 AM
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Default Re: Protein Purification Problem

which column did you use? in case of ni-nta, >250mM imidazole should work.
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Old 12-22-2008, 05:02 PM
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Default Re: Protein Purification Problem

Hi

I recomend you to read challenging proteins from Amersham
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The Following User Says Thank You to ednot For This Useful Post:
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Old 08-16-2009, 08:59 AM
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Default Re: Protein Purification Problem

Thanks for the info, I appreciate it.
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