Protein Purification Problem

Tamson · #1 12-28-2007, 11:45 PM

Hello,
I have his-tagged my protein however I am having problems getting it off my column.

Any ideas what is going on?

cheers

angie80 · #2 01-03-2008, 04:23 AM

which column did you use? in case of ni-nta, >250mM imidazole should work.

ednot · #3 12-22-2008, 04:02 PM

Hi

I recomend you to read challenging proteins from Amersham
[Only registered users see links. ]

[Only registered users see links. ]

[Only registered users see links. ]

Cool man · #4 08-16-2009, 08:59 AM

Thanks for the info, I appreciate it.

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Protein Purification Problem

Protein Purification Problem - Protein Science

Protein Purification Problem - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

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