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Tamson 12-29-2007 12:45 AM

Protein Purification Problem
 
Hello,
I have his-tagged my protein however I am having problems getting it off my column.

Any ideas what is going on?

cheers

angie80 01-03-2008 05:23 AM

Re: Protein Purification Problem
 
which column did you use? in case of ni-nta, >250mM imidazole should work.

ednot 12-22-2008 05:02 PM

Re: Protein Purification Problem
 
Hi

I recomend you to read challenging proteins from Amersham
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Cool man 08-16-2009 08:59 AM

Re: Protein Purification Problem
 
Thanks for the info, I appreciate it.


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