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- - Protein Purification Problem (http://www.molecularstation.com/forum/protein-science/2835-protein-purification-problem.html)

Tamson

12-28-2007 11:45 PM

Protein Purification Problem

Hello,
I have his-tagged my protein however I am having problems getting it off my column.

Any ideas what is going on?

cheers

angie80

01-03-2008 04:23 AM

Re: Protein Purification Problem

which column did you use? in case of ni-nta, >250mM imidazole should work.

ednot

12-22-2008 04:02 PM

Re: Protein Purification Problem

Hi

I recomend you to read challenging proteins from Amersham
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Cool man

08-16-2009 08:59 AM

Re: Protein Purification Problem

Thanks for the info, I appreciate it.

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