		Molecular Biology Forum > Molecular Research Topics Forum > Protein Science
Protein Purification Problem

Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

Protein Science

by kiki06
· · ·
Protein Pictures
16 photos
9 comments

by admin
· · ·
Protein Pictures
16 photos
9 comments

Protein Purification Problem

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

LinkBack

Thread Tools

Display Modes

#1 (permalink)

12-28-2007, 11:45 PM

Tamson

Pipette Filler

Join Date: Dec 2007

Posts: 1

Protein Purification Problem

Hello,
I have his-tagged my protein however I am having problems getting it off my column.

Any ideas what is going on?

cheers

Today

Advertising
Google Adsense

This advertising will not be shown

in this way to registered members.

and become a member on

Molecular Biology Forum

Sponsored Links

#2 (permalink)

01-03-2008, 04:23 AM

angie80

Pipette Filler

Join Date: Jan 2008

Posts: 1

Re: Protein Purification Problem

which column did you use? in case of ni-nta, >250mM imidazole should work.

Tags

protein, purification

« Difference between Western Blotting and Immunoprecipitation? | iHOP retrieves every sentence in PUBMED that cites your protein of interest. »

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are On Pingbacks are On Refbacks are On

Forum Jump

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
His Tag Protein Purification Problems	IoDiNE	Recombinant Protein Forum	1	02-26-2008 02:22 PM
Production of Monoclonal Antibodies - Technique in Mouse	Cellbiogal	Antibody Forum	1	02-17-2008 02:00 PM
His-tag protein purification under denaturing conditions	Arabidop	Molecular Biology Articles and Protocols	3	11-27-2007 10:00 PM
Protein Purification Course: Principles and Practice (Jan 07)	kiki06	Conferences , Symposiums and Meetings	0	11-30-2006 08:36 PM
A protocol for GST preparation and protein purification	Arabidop	Molecular Biology Articles and Protocols	1	11-29-2006 01:29 PM

All times are GMT. The time now is 10:31 AM.

Member Login

Protein Purification Problem

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.