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Monomer Dimer Separation Problems on SDS-PAGE

Monomer Dimer Separation Problems on SDS-PAGE - Protein Science

Monomer Dimer Separation Problems on SDS-PAGE - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 11-26-2007, 08:34 AM
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Question Monomer Dimer Separation Problems on SDS-PAGE



Hello everyone, I need some help as I do SDS-PAGE with whole cell lysates in order to visualize my protein, however it forms monomers within the cytoplasm but upon ligand binding, it dimerizes and is translocates into the nucleus.


If I treat my cells with SDS loading buffer, will it be enough to lyse both the cell membrane and the nucleus membrane, to allow proteins from both the nucleus and cytoplasm to be assayed?

Would it be a better idea to extract the nucleus from the cytoplasm fraction and analyze the fractions then separately on an SDS-PAGE gel?

cheers
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