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extraction of collagen I from cells

extraction of collagen I from cells - Protein Science

extraction of collagen I from cells - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 11-21-2007, 07:02 PM
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Default extraction of collagen I from cells



I am looking for a protocol that will effectively extract collagen I from uterine smooth muscle cells grown in culture. My goal is to collect both the pro-collagen and mature collagen from the cells as well as the collagen that becomes attached to the plastic dish during culturing. Does anyone have a suggestions on how to approach this? Thanks!
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