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Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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protein-synthesis-epic-cellular-level Protein Synthesis Epic Cellular Level. Directed in 1971 by Robert Alan Weiss for the Department of Chemistry of Stanford University and imprinted with the free love aura of the period this short film continues to be shown in biology class today. It has since spawn a series of similar funny attempts at vulgarizing protein synthesis. Narrated by Paul Berg 1980 Nobel prize for Chemistry.

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extraction of collagen I from cells

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.



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Old 11-21-2007, 06:02 PM
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Default extraction of collagen I from cells

I am looking for a protocol that will effectively extract collagen I from uterine smooth muscle cells grown in culture. My goal is to collect both the pro-collagen and mature collagen from the cells as well as the collagen that becomes attached to the plastic dish during culturing. Does anyone have a suggestions on how to approach this? Thanks!
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