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-   -   extraction of collagen I from cells (http://www.molecularstation.com/forum/protein-science/2453-extraction-collagen-i-cells.html)

kimobelton 11-21-2007 07:02 PM

extraction of collagen I from cells
 
I am looking for a protocol that will effectively extract collagen I from uterine smooth muscle cells grown in culture. My goal is to collect both the pro-collagen and mature collagen from the cells as well as the collagen that becomes attached to the plastic dish during culturing. Does anyone have a suggestions on how to approach this? Thanks!


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