I am trying to get specific interactions of two protein using GST pull down assays.
However the problem is that i get a band in the GST control sample.
I did GST pull-down assay to see if my GST-fused protein really binds to another protein in the cell lysate.
- Basic GST Pull Down Protocol
- GST Pull down assay
- Protein elution with Laemmli buffer
- Western Blotting
I know that BSA can block the non-specific binding of protein to beads.
however i didnt block for feat that the BSA would show up everywhere.
Should I block by using bovine serum albumin (BSA) or another method?
Notes on the GST pull down.
I had pre-cleared the cell lysates with GST and glutathione agarose beads.
How can I eliminate this non-specific bands?