Protein Quantification

sazza7 · #1 (**permalink**) 11-04-2007, 06:18 PM

Hello!
For protein quantification I wanted to use the 2D-Quant Kit (GE), but had some problems with it. There is one thing I really cannot explain: How can I get several totally different protein concentrations by using just different dilutions of my protein solution?? Does anyone exist who also uses this kit? Or what`s your favoured method for protein quantification?

Sazza7 - Michigan

danfive · #2 (**permalink**) 11-04-2007, 06:29 PM

Perhaps the absorbance range of your dilution series does not fall within the standards' range. Or the sample dilution may also be diluting detergents, buffer salts, etc., that may make a difference. I usually use a 1:20 dilution and don't have any problems. I always prefer protein kits from Pierce like the protein microassay.

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Protein Quantification

Protein Science

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