Recombinant Protein Expression Systems

domba · #1 (**permalink**) 08-01-2006, 12:07 AM

Hey!
Which are the best recombinant protein expression systems for generation of
Antibodies?
Enzyme activity?

thanks

kiki06 · #2 (**permalink**) 10-03-2006, 05:46 PM

Hey Domba,
I guess it depends what you want to do with the protein as you mention.

The best system for keeping post-translational modifications / folding of the native protein ie ENZYME ACTIVITY would be a eukaryotic system such as yeast, or more preferrably insect (bacculovirus), or even better a mammalian system.

The best system for antibody production would be the bacterial (easy to grow) however inclusion body purification may be a problem.

Seriously, I would go with Yeast as you can tag your protein to be expressed in the media, and therefore you can just collect media and keep your yeast producing protein.
Dont forget to tag your protein and also allow cleavage of the tag. Copper or smaller tags are better than bigger tags.

Cheers

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