SDS-PAGE Polymerization Troubleshooting

[DNA_science]

Hello all, I recently am pouring SDS-PAGE gels when a liquid layer develops on top of the gel.

This happens in the resolving and stacking gel layers.

The liquid layer is denser than even butanol I add to the top of my gels.

I have purchased new Acrylamide-bis mixture (30%/.8%), even 10% APS, and also TEMED solutions.

However the liquid layer appears again and polymerization takes 2 hours

Has anyone seen this before and how do I stop it?

thanks
DNA Science

[shweta]

hi...ys u r rght...m also facing the same problem.....as wid u...whether i have made this before some days as now a days proceed....i also changed the solutions....bt also having problem in polymerization to takmore tim....i think the handing and pipetting steps lik factors may b responcible here...