SDS-PAGE Polymerization Troubleshooting

DNA_science · #1 (**permalink**) 10-26-2007, 07:24 AM

Hello all, I recently am pouring SDS-PAGE gels when a liquid layer develops on top of the gel.

This happens in the resolving and stacking gel layers.

The liquid layer is denser than even butanol I add to the top of my gels.

I have purchased new Acrylamide-bis mixture (30%/.8%), even 10% APS, and also TEMED solutions.

However the liquid layer appears again and polymerization takes 2 hours

Has anyone seen this before and how do I stop it?

thanks
DNA Science

shweta · #2 (**permalink**) 11-27-2007, 03:48 PM

hi...ys u r rght...m also facing the same problem.....as wid u...whether i have made this before some days as now a days proceed....i also changed the solutions....bt also having problem in polymerization to takmore tim....i think the handing and pipetting steps lik factors may b responcible here...

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SDS-PAGE Polymerization Troubleshooting

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