Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science
Register Search Today's Posts Mark Forums Read

Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


removal of enterokinase

removal of enterokinase - Protein Science

removal of enterokinase - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-23-2007, 01:28 PM
Pipette Filler
Points: 1,457, Level: 22 Points: 1,457, Level: 22 Points: 1,457, Level: 22
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2007
Posts: 6
Thanks: 0
Thanked 0 Times in 0 Posts
Default removal of enterokinase



I have cloned gene for a protein in pET32a vector so that the protein gets expressed along with his tag and thioredoxin tag which can be cleaved off with enterokinase from my protein after purification on Ni-NTA column .Can any one suggest how i can separate the enterokinase from my protein after digestion? I am aware of enterokinase capture columns of sigma and invitrogen,But i cannot afford to buy them. I am using NEB's light chain enterokinase.
Reply With Quote
Reply

Tags
enterokinase , removal


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
DNA removal used in your Laboratory butters PCR - Polymerase Chain Reaction Forum 1 09-28-2009 04:14 AM
[Yeast] TEM procedure using cell wall removal! Z.J. Zhang Yeast Forum 0 09-01-2006 05:30 PM
Removal of external bacteria from C. elegans Sarah Perkins C Elegans Forum 0 06-12-2006 01:41 PM
Removal of protein from glycogen extractant? Ngo Thi Thuy Huong Protocols and Methods Forum 1 03-27-2006 05:22 PM


All times are GMT. The time now is 09:39 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10898 seconds with 16 queries