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Recombinant Protein Gel Filtration Contamination Removal

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Recombinant Protein Gel Filtration Contamination Removal

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

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09-07-2007, 10:04 AM

oBWhat

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Join Date: May 2006

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Recombinant Protein Gel Filtration Contamination Removal

our protein is a recombinant ecoli based protein with mol wt 20 kDa. it contains high molecular weight contaminating proteins which we desire to remove. we
have superdex 75 prep grade gel filtration media that is recommended for recombinant proteins.
can anyone guide me on the feed volume concentration etc for gel filtration or a suitable protocol for the same?

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Recombinant Protein Gel Filtration Contamination Removal

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.