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Recombinant Protein Gel Filtration Contamination Removal

Recombinant Protein Gel Filtration Contamination Removal - Protein Science

Recombinant Protein Gel Filtration Contamination Removal - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 09-07-2007, 10:04 AM
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Question Recombinant Protein Gel Filtration Contamination Removal



our protein is a recombinant ecoli based protein with mol wt 20 kDa. it contains high molecular weight contaminating proteins which we desire to remove. we
have superdex 75 prep grade gel filtration media that is recommended for recombinant proteins.
can anyone guide me on the feed volume concentration etc for gel filtration or a suitable protocol for the same?
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