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question about protein solubilization

question about protein solubilization - Protein Science

question about protein solubilization - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 08-27-2007, 02:58 PM
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Default question about protein solubilization



Will anything happen to a set of protein samples that were lyophilized to dryness in a microtiter plate if they were left overnight at room temperature in a solution of 10% ACN and ammonium bicarbonate? Typically, they need to be stored away at 4 degrees Celsius, but the mistake was already made, and wondering if they have degraded or become unstable???

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Old 08-27-2007, 02:58 PM
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Default Re: question about protein solubilization

You are fine. I assume the lyophilized samples are in solution now, but acetonitrile and ammonium bicarbonate won't harm, degrade your protein.
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