question about protein solubilization

molbiol · #1 (**permalink**) 08-27-2007, 02:58 PM

Will anything happen to a set of protein samples that were lyophilized to dryness in a microtiter plate if they were left overnight at room temperature in a solution of 10% ACN and ammonium bicarbonate? Typically, they need to be stored away at 4 degrees Celsius, but the mistake was already made, and wondering if they have degraded or become unstable???

posted by molbio123 (please re-register)

danfive · #2 (**permalink**) 08-27-2007, 02:58 PM

You are fine. I assume the lyophilized samples are in solution now, but acetonitrile and ammonium bicarbonate won't harm, degrade your protein.

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question about protein solubilization

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