trouble with EMSA?

Henry Chen · #1 (**permalink**) 08-27-2007, 01:19 PM

Hi,
At present, I am doing EMSA experiments with NF-kB, but I find it did not work although I have optimized the conditions. Any suggestions are welcomed. Thanks a lot.

kmunson779 · #2 (**permalink**) 08-27-2007, 04:42 PM

"It doesn't work" isn't very descriptive. Do you mean that you can see probe on the gel but you can't see a shift when you add your protein/extract? Or that you get a shift but you can't supershift it with an antibody or see a change upon drug treatment? Or that you can't see your probe on the gel at all?

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