Coomassie Brilliant Blue in Bradford's assay

cchivy · #1 (**permalink**) 08-27-2007, 12:03 PM

Can I use Coomassie Brilliant Blue (CBB) R-250 in my Bradford's assay protein estimation instead of the usual CBB G-250? Doesn't sound like a crime to me, but will I get punishing-ly weird results??

I asked this because I have only CBB R-250 in my lab now

kmunson779 · #2 (**permalink**) 08-27-2007, 04:50 PM

I think the properties of G and R are sufficiently different that it won't work... but you could always try to do a standard curve with BSA and see if I'm wrong.

cchivy · #3 (**permalink**) 08-28-2007, 02:48 PM

Thanks Kmunson779. I'll try out with BSA standard curve to check.

THanks

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Coomassie Brilliant Blue in Bradford's assay

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