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#1
08-27-2007, 12:03 PM
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 Coomassie Brilliant Blue in Bradford's assay Can I use Coomassie Brilliant Blue (CBB) R-250 in my Bradford's assay protein estimation instead of the usual CBB G-250? Doesn't sound like a crime to me, but will I get punishing-ly weird results?? I asked this because I have only CBB R-250 in my lab now  |
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#2
08-27-2007, 04:50 PM
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 Re: Coomassie Brilliant Blue in Bradford's assay I think the properties of G and R are sufficiently different that it won't work... but you could always try to do a standard curve with BSA and see if I'm wrong. |
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| assay , blue , bradford , brilliant , coomassie |
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