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Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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protein-synthesis-epic-cellular-level Protein Synthesis Epic Cellular Level. Directed in 1971 by Robert Alan Weiss for the Department of Chemistry of Stanford University and imprinted with the free love aura of the period this short film continues to be shown in biology class today. It has since spawn a series of similar funny attempts at vulgarizing protein synthesis. Narrated by Paul Berg 1980 Nobel prize for Chemistry.

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Coomassie Brilliant Blue in Bradford's assay

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.



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  #1 (permalink)  
Old 08-27-2007, 12:03 PM
Pipette Filler
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Question Coomassie Brilliant Blue in Bradford's assay

Can I use Coomassie Brilliant Blue (CBB) R-250 in my Bradford's assay protein estimation instead of the usual CBB G-250? Doesn't sound like a crime to me, but will I get punishing-ly weird results??

I asked this because I have only CBB R-250 in my lab now
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  #2 (permalink)  
Old 08-27-2007, 04:50 PM
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Default Re: Coomassie Brilliant Blue in Bradford's assay

I think the properties of G and R are sufficiently different that it won't work... but you could always try to do a standard curve with BSA and see if I'm wrong.
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Old 08-28-2007, 02:48 PM
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Default Re: Coomassie Brilliant Blue in Bradford's assay

Thanks Kmunson779. I'll try out with BSA standard curve to check.

THanks
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