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Coomassie Brilliant Blue in Bradford's assay

Coomassie Brilliant Blue in Bradford's assay - Protein Science

Coomassie Brilliant Blue in Bradford's assay - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 08-27-2007, 12:03 PM
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Question Coomassie Brilliant Blue in Bradford's assay



Can I use Coomassie Brilliant Blue (CBB) R-250 in my Bradford's assay protein estimation instead of the usual CBB G-250? Doesn't sound like a crime to me, but will I get punishing-ly weird results??

I asked this because I have only CBB R-250 in my lab now
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Old 08-27-2007, 04:50 PM
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Default Re: Coomassie Brilliant Blue in Bradford's assay

I think the properties of G and R are sufficiently different that it won't work... but you could always try to do a standard curve with BSA and see if I'm wrong.
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Old 08-28-2007, 02:48 PM
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Default Re: Coomassie Brilliant Blue in Bradford's assay

Thanks Kmunson779. I'll try out with BSA standard curve to check.

THanks
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