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Refolding Protein Buffers

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protein-synthesis-epic-cellular-level Protein Synthesis Epic Cellular Level. Directed in 1971 by Robert Alan Weiss for the Department of Chemistry of Stanford University and imprinted with the free love aura of the period this short film continues to be shown in biology class today. It has since spawn a series of similar funny attempts at vulgarizing protein synthesis. Narrated by Paul Berg 1980 Nobel prize for Chemistry.

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Refolding Protein Buffers

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

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08-27-2007, 11:45 AM

tonyz06

Pipette Filler

Join Date: Feb 2006

Posts: 18

Refolding Protein Buffers

Hi, I am trying to refold proteins that I isolated from isolation bodies in bacteria (e.coli that are insoluble aggregates).

Unfortunately, my proteins seem to not have activity. What buffers can I use to try and refold my proteins back to functional form?

any ideas?
cheers

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Refolding Protein Buffers

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.