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Refolding Protein Buffers

Refolding Protein Buffers - Protein Science

Refolding Protein Buffers - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 08-27-2007, 11:45 AM
Pipette Filler
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Question Refolding Protein Buffers



Hi, I am trying to refold proteins that I isolated from isolation bodies in bacteria (e.coli that are insoluble aggregates).

Unfortunately, my proteins seem to not have activity. What buffers can I use to try and refold my proteins back to functional form?

any ideas?
cheers
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