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-   -   Refolding Protein Buffers (http://www.molecularstation.com/forum/protein-science/1866-refolding-protein-buffers.html)

tonyz06 08-27-2007 11:45 AM

Refolding Protein Buffers
 
Hi, I am trying to refold proteins that I isolated from isolation bodies in bacteria (e.coli that are insoluble aggregates).

Unfortunately, my proteins seem to not have activity. What buffers can I use to try and refold my proteins back to functional form?

any ideas?
cheers:mad:


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