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An odd-looking Michaelis-Menten curve

An odd-looking Michaelis-Menten curve - Protein Science

An odd-looking Michaelis-Menten curve - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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Old 07-27-2007, 12:35 AM
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Smile An odd-looking Michaelis-Menten curve



Hi all,

I have been assaying an enzyme that oxidizes OH groups and reduces NADP+. The assay is based on the absorbance of NADPH at 340 nm.

The problem is that the graph of reaction rate versus substrate concentration looks like an exponential curve rather than the usual Michaelis-Menten curve I have obtained for other mutants.

Has anyone encountered this problem? I've tried using freshly purified enzyme, changing the amount of enzyme and NADP+.

Thanks in advance!
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