		Molecular Biology Forum > Molecular Research Topics Forum > Protein Science
An odd-looking Michaelis-Menten curve

Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

protein Videos

protein-synthesis-epic-cellular-level Protein Synthesis Epic Cellular Level. Directed in 1971 by Robert Alan Weiss for the Department of Chemistry of Stanford University and imprinted with the free love aura of the period this short film continues to be shown in biology class today. It has since spawn a series of similar funny attempts at vulgarizing protein synthesis. Narrated by Paul Berg 1980 Nobel prize for Chemistry.

Protein Science

by admin
· · ·
Protein Pictures
17 photos
10 comments

by tomms
· · ·
Protein Pictures
17 photos
10 comments

by admin
· · ·
Protein Pictures
17 photos
10 comments

An odd-looking Michaelis-Menten curve

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.

LinkBack

Thread Tools

Display Modes

#1 (permalink)

07-27-2007, 12:35 AM

jak3099

Pipette Filler

Join Date: Jul 2007

Posts: 1

An odd-looking Michaelis-Menten curve

Hi all,

I have been assaying an enzyme that oxidizes OH groups and reduces NADP+. The assay is based on the absorbance of NADPH at 340 nm.

The problem is that the graph of reaction rate versus substrate concentration looks like an exponential curve rather than the usual Michaelis-Menten curve I have obtained for other mutants.

Has anyone encountered this problem? I've tried using freshly purified enzyme, changing the amount of enzyme and NADP+.

Thanks in advance!

Today

Advertising
Google Adsense

This advertising will not be shown

in this way to registered members.

and become a member on

Molecular Biology Forum

Sponsored Links

« not expert in electrophoresis | Refolding Protein Buffers »

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are On Pingbacks are On Refbacks are On

Forum Jump

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Standard Curve	CPPnerds	Real-Time PCR and Quantitative PCR Forum	1	04-09-2008 05:45 PM
Standard curve	mvermehren	Real-Time PCR and Quantitative PCR Forum	4	07-27-2007 06:02 PM

All times are GMT. The time now is 02:42 AM.

Member Login

An odd-looking Michaelis-Menten curve

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.