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An odd-looking Michaelis-Menten curve
I have been assaying an enzyme that oxidizes OH groups and reduces NADP+. The assay is based on the absorbance of NADPH at 340 nm.
The problem is that the graph of reaction rate versus substrate concentration looks like an exponential curve rather than the usual Michaelis-Menten curve I have obtained for other mutants.
Has anyone encountered this problem? I've tried using freshly purified enzyme, changing the amount of enzyme and NADP+.
Thanks in advance!
|curve , michaelismenten , oddlooking|
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