Immunoprecipitation Controls

oBWhat · #1 (**permalink**) 06-29-2007, 08:47 PM

Hello all,
I was looking for how to make controls for IP. What do you guys use?

Cheers

danfive · #2 (**permalink**) 07-17-2007, 08:15 PM

Recombinant protein controls are the best, but hard to come by in my research. So I use cell lysate from an overexpressing cell line. Spin column "purification" to simplify lysate--keep correct protein size range, (dilute it accordingly)--dump the rest.

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Immunoprecipitation Controls

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