Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science
Register Search Today's Posts Mark Forums Read

Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


Immunoprecipitation Controls

Immunoprecipitation Controls - Protein Science

Immunoprecipitation Controls - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-29-2007, 08:47 PM
oBWhat's Avatar
Graduate Student
Points: 3,826, Level: 41 Points: 3,826, Level: 41 Points: 3,826, Level: 41
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2006
Posts: 124
Thanks: 0
Thanked 7 Times in 7 Posts
Question Immunoprecipitation Controls



Hello all,
I was looking for how to make controls for IP. What do you guys use?

Cheers
Reply With Quote
  #2  
Old 07-17-2007, 08:15 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Default Re: Immunoprecipitation Controls

Recombinant protein controls are the best, but hard to come by in my research. So I use cell lysate from an overexpressing cell line. Spin column "purification" to simplify lysate--keep correct protein size range, (dilute it accordingly)--dump the rest.
Reply With Quote
Reply

Tags
controls , immunoprecipitation


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
test if there is a difference between cases and controls SSN BioStatistics Forum 0 12-21-2009 02:38 AM
Internal Microarray Controls? albinpaul Microarrays Forum 0 07-23-2008 12:12 PM
Essential Immunoprecipitation controls??? Orolicki, Slobodanka Cell Biology and Cell Culture 0 02-09-2007 03:08 PM
The Importance of Proper Controls admin Science News and Views 1 01-02-2007 05:01 AM
problem with a negative controls Anna Protocols and Methods Forum 4 08-01-2004 06:51 AM


All times are GMT. The time now is 04:49 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11735 seconds with 16 queries