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#1
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| Hello all, I was looking for how to make controls for IP. What do you guys use? Cheers |
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#2
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| Recombinant protein controls are the best, but hard to come by in my research. So I use cell lysate from an overexpressing cell line. Spin column "purification" to simplify lysate--keep correct protein size range, (dilute it accordingly)--dump the rest. |
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| controls , immunoprecipitation |
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| Thread | Thread Starter | Forum | Replies | Last Post |
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