not expert in electrophoresis

[waffaa_qm]

hi
Iam asking about running buffer
If Iput it in blastic tube until next day is it harmfull to the ruuning buffer
and can any expert analyse my result of gel which attached

[danfive]

Running buffer is very simple solution, plastic container won't hurt it. Gel looks fine--what are you trying to get? Third lane looks overloaded, fifth and seventh lane look underloaded.

[waffaa_qm]

thank you very much for your reply
I make identification of meat according to species
so what is the solution of deformaty lanes and I can`t repeat this work again
Is this picture accepted in discussion
thanks a lot

[danfive]

Do you need to get uniform protein concentration across all the lanes on your gel? I am not sure what you mean exactly. But if you are looking to improve your sample loading on gel follow these guidelines: 1. Treat all your samples in the same manner---homogenize in the same way, centrifuge exactly the same way everytime, remove supernatant to new tube same way (preferably without touching or transfering any pellet). 2. Determine protein concentration the same way (like Pierce BCA protein assay), dilute in same dilution buffer at same ratio (1:10 or 1:40, for example), freeze or store on ice, the same way. 3. Calculate the volume that results in the exact same amount of total protein to load (like loading 40ug or 80ug) for each lane. 4. Finally before loading, prep the samples the same way, use the same loading buffer (include or exclue BME or DTT), heat 5 min, centrifuge quickly, load. 5. Run the same parameters for your gel the same, everytime, like 80V for 60 min, in Tris-glycine-SDS running buffer.