PAGE beginner

waffaa_qm · #1 (**permalink**) 04-21-2007, 12:31 AM

Iam beginner in learning SDS PAGE
Iam in master degree
my problem is when I work with gel 12% get good bands but if I work with gel 6% the bands is very light even I put the same concentration of sample in wells in the two gel
so what is the problem
And how can I analyze the bands by computer
please anyone help me
Thanks

mara1412 · #2 (**permalink**) 04-24-2007, 11:48 AM

Hi,
what is the molecular weight of your protein of interest? You've got to choose the right acrylamide concentration for your protein. If you want to see good bands for heavy proteins (> 75kda) you should use 6-8% acrilamyde gel, while if your protein of interest is not so big(< 75kda) the right concentration should be between 9-12 %.
Mara

waffaa_qm · #3 (**permalink**) 04-24-2007, 09:27 PM

hi mara Thank you very much for your replying
and I didnot search for certain protein . my research is identifying the meat according to species by electrophoresis
so do you have any recommindation and how can I analyze the photo of the gel
please reply soon
thanks

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Convex shape of sample bands on SDS PAGE gel	ssacket1	Western Blot Forum	4	12-04-2007 12:09 AM
Peptide sequencing from old SDS PAGE gel	Brownie	Peptide Forum	0	09-17-2007 07:10 PM
New Resource Page for Life Scientists	lsutton	Protocols and Methods Forum	0	07-18-2007 06:36 PM
Update! New Scientist Profiles Page in Progress	admin	Molecular Biology News and Announcements	1	07-15-2007 03:59 AM
Our RNAi and SiRNA Page is Now Open!	admin	RNAi and SiRNA Forum	0	01-03-2006 07:34 PM

Member Login

PAGE beginner

Protein Science

Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.