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Protein Science Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


protein preservation

protein preservation - Protein Science

protein preservation - Post Questions and Discuss Protein Structure and Function, Protein Localization etc.. Please do not post Proteomic questions here, there is a Proteomic Forum below.


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  #1  
Old 06-23-2006, 01:00 PM
aftabac's Avatar
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Default protein preservation



Hi dear

i have purified my protein that is a recombinant protein and now i want to preserve it, what is the best way and buffer for protein preservation of short and long time

i hope you will guide me

best wishes
aftab ahmad
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Old 06-26-2006, 06:13 PM
Pipette Filler
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Default Re: protein preservation

Hey Aftabac,

Protein preservation is best at -80C but make sure you store in MANY aliquots.

This way you do not freeze and thaw many times. Freeze thawing denatures the proteins and activity of the protein is lost.

Storing short term (a week or a few days) -20C is ok.
Do not store protein even for short term in 4C.

Buffer I guess does not matter as long as it is buffered and protein activity will not be affected in the buffer chosen.

Ciao!
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Old 12-12-2012, 06:58 AM
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Default Re: protein preservation

I preserve the protein is always good for our body and gaining the body specailly those people who make to the strong and powerful and gaining the body that people is very helpful for the supplement and useful, i also use the protein and i am a strong.
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