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Radioligand Saturation Assay HELP!!

Radioligand Saturation Assay HELP!! - Protein Forum

Radioligand Saturation Assay HELP!! - Protein Forum


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Old 06-04-2012, 07:43 PM
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Default Radioligand Saturation Assay HELP!!



I want to do a saturation assay using a single conc of protein and varying conc of radioligand.

I'm sure how to vary the radioligand conc without changing the radioactivity. (i.e. I need 190Bq/10 ul for each conc). Do I dilute with unlabelled ligand? How does this affect binding and the cpm/dpm I can measure????
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