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#1
06-04-2012, 07:43 PM
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 Radioligand Saturation Assay HELP!! I want to do a saturation assay using a single conc of protein and varying conc of radioligand. I'm sure how to vary the radioligand conc without changing the radioactivity. (i.e. I need 190Bq/10 ul for each conc). Do I dilute with unlabelled ligand? How does this affect binding and the cpm/dpm I can measure????  |
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| assay , radioligand , saturation |
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