Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum

Removing Albumin from Samples

Removing Albumin from Samples - Protein Forum

Removing Albumin from Samples - Protein Forum

LinkBack Thread Tools Display Modes
Old 01-03-2012, 08:03 PM
Pipette Filler
Points: 89, Level: 1 Points: 89, Level: 1 Points: 89, Level: 1
Activity: 0% Activity: 0% Activity: 0%
Join Date: Jan 2012
Posts: 5
Thanks: 3
Thanked 0 Times in 0 Posts
Default Removing Albumin from Samples

Hey guys, I'm trying to analyze media that I've collected from growing cells for fluctuating levels of my protein of interest. Unfortunately, when I run my Western Blots, I get this fat streak/band of albumin which obscures any band I would possible be able to see. I was wondering if anyone has any ideas on the best way to remove albumin from my samples so that I can get clean WB results.
Reply With Quote
Old 02-26-2012, 04:39 PM
Zagami's Avatar
Ph.D Doctorate
Points: 4,488, Level: 45 Points: 4,488, Level: 45 Points: 4,488, Level: 45
Activity: 50% Activity: 50% Activity: 50%
Join Date: Sep 2009
Location: Tabaka Mission Hospital - Kiisi -Kenya & Italy Sicily
Posts: 171
Thanks: 2
Thanked 28 Times in 24 Posts
Default Re: Removing Albumin from Samples

see you my post on [Only registered users see links. ]
Reply With Quote

albumin , removing , samples

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Dead staining... some samples work others don't PLEASE HELP! bigdmajor Immunofluorescence Forum 6 03-10-2010 09:30 PM
removing albumin pirouz Protein Science 8 09-30-2009 04:57 AM
Remove Albumin Contamination from Purification Protein proatpcr Recombinant Protein Forum 1 11-17-2008 10:59 PM
removing bound SDS from protein samples (Larisa) Tay liang xin Protocols and Methods Forum 0 12-28-2007 03:55 AM
removing bound SDS from protein samples Larisa Protocols and Methods Forum 1 12-18-2007 02:43 AM

All times are GMT. The time now is 08:44 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11569 seconds with 16 queries