Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum


Protein characterization

Protein characterization - Protein Forum

Protein characterization - Protein Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-28-2011, 10:16 AM
UnSubscribed Vendor Advertiser
Points: 172, Level: 3 Points: 172, Level: 3 Points: 172, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2011
Location: Springfield, VA, USA
Posts: 11
Thanks: 0
Thanked 0 Times in 0 Posts
Default Protein characterization



Hi all!

Proteins are digested prior to shotgun mass spectrometry on a state-of-the-art LTQ Velos tandem mass spectrometer (LC-MS/MS). Data is analyzed by up to four different search algorithms. Iterative searches are performed to maximize sequence coverage of identified proteins. This method is capable of identifying over 1,000 proteins in a complex mixture, however, sequence coverage will improve as the number of proteins decreases or enrichment of a set of proteins increases.

Protein Characterization can include searches of:
- custom sequences,
- post-translational modifications (including known, previously characterized modifications),
- known single nucleotide polymorphisms (SNPs),
- unexpected amino acid point mutations.

Yuriy
Reply With Quote
  #2  
Old 12-08-2011, 05:34 AM
Pipette Filler
Points: 2, Level: 1 Points: 2, Level: 1 Points: 2, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Protein characterization

Thanks for explaining the topic.
I got the topic in detail. I was looking
for it from many days.
Reply With Quote
  #3  
Old 02-11-2013, 06:46 AM
Summer Student
Points: 260, Level: 5 Points: 260, Level: 5 Points: 260, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2013
Posts: 54
Thanks: 0
Thanked 0 Times in 0 Posts
Default ジョーダン 2013

[

[

[
Reply With Quote
Reply

Tags
characterization , mass spectrometry , protein , proteomic


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM


All times are GMT. The time now is 01:05 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.17254 seconds with 16 queries