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NBT oxidation

NBT oxidation - Protein Forum

NBT oxidation - Protein Forum


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Old 04-05-2011, 07:58 PM
Pipette Filler
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Default NBT oxidation



I made measurement with serinol (2-amino-1,3-propanediol): added supernatant of cell lysis after sonification, NADH and nitroblue tetrazolium salt (which reduced gives purple color). And that gave me reduced NBT. I do not understood how reduced NBT, and what was oxidated in serinol. Without NBT or NADH was no activity. Anyone can help me with that mechanism of oxidation/reduction reaction.
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