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protein concentration decrease after protein purification

protein concentration decrease after protein purification - Protein Forum

protein concentration decrease after protein purification - Protein Forum


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Old 03-20-2011, 03:36 AM
Pipette Filler
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Unhappy protein concentration decrease after protein purification



Hi,

I did purification (his tag) by FPCL after that I did dialysis

I need injection for rabbit but a protein concentration decrease to 97 g/ml. it

is not enough For injection

Do you know what I can do?



Thank you
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Old 09-18-2012, 03:01 AM
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Default Re: protein concentration decrease after protein purification

Can I know where do you come from?
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Old 10-22-2012, 07:12 PM
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Default Re: protein concentration decrease after protein purification

Sounds like you might have to make more! Also you can try concentrating your protein (Amplicon with 10 kDa cut off usually works depends on the size of your protein).
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