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#1
11-23-2010, 10:37 AM
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 Deglycosylation of proteins Hi My protein is predicted to be glycosylated I have bought enzymes from Fermentas to de-glycosylate it My question is about running the proteins on a gel afterwards to see a difference in size.... The protocol states to denature the proteins for 10 mins at 100 degrees then incubate with the enzymes for 1-4h at 37 degrees Do I then need to re-boil after addition of sample buffer before running on an SDS-Page gel to see if the size of the protein has been decreased? Ta  |
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