Deglycosylation of proteins
My protein is predicted to be glycosylated
I have bought enzymes from Fermentas to de-glycosylate it
My question is about running the proteins on a gel afterwards to see a difference in size....
The protocol states to denature the proteins for 10 mins at 100 degrees then incubate with the enzymes for 1-4h at 37 degrees
Do I then need to re-boil after addition of sample buffer before running on an SDS-Page gel to see if the size of the protein has been decreased?
|deglycosylation , proteins|
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