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Deglycosylation of proteins

Deglycosylation of proteins - Protein Forum

Deglycosylation of proteins - Protein Forum


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Old 11-23-2010, 11:37 AM
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Default Deglycosylation of proteins



Hi
My protein is predicted to be glycosylated
I have bought enzymes from Fermentas to de-glycosylate it
My question is about running the proteins on a gel afterwards to see a difference in size....

The protocol states to denature the proteins for 10 mins at 100 degrees then incubate with the enzymes for 1-4h at 37 degrees

Do I then need to re-boil after addition of sample buffer before running on an SDS-Page gel to see if the size of the protein has been decreased?

Ta
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Old 11-23-2010, 02:33 PM
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Default Re: Deglycosylation of proteins

Yes, boil 5min in laemmli buffer.
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