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silver staining ethanol Tris-Tricine gels

silver staining ethanol Tris-Tricine gels - Protein Forum

silver staining ethanol Tris-Tricine gels - Protein Forum


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Old 07-22-2010, 01:06 PM
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Default silver staining ethanol Tris-Tricine gels



Hi all!
Can anyone suggest a silver staining protocol for Tris-Tricine gels where ethanol is used instead of methanol (with a fixation step to prevent low-weight peptides from washing out). And are Tricine gels different from more conventional Glycine gels in terms of Ag concentration needed for staining? Thanks in advance.
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