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Capillary coating in CE.

Capillary coating in CE. - Protein Forum

Capillary coating in CE. - Protein Forum


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Old 07-20-2010, 05:50 PM
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Default Capillary coating in CE.



Hi,

I have been trying to run some basic proteins through a capillary but they get stuck on the capillary wall. I have tried one of the numerous capillary coating protocols (a triple layer with polybrene to make the capillary positively charged) available but it does not last long. I had to repeat the coating every 10 runs! Are there any more permanent capillary coatings?

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