I doing some Luciferase assays (Promega).
But after reading the luciferase I want to recover all the protein extract (that is diluted on Passive Lysis Buffer from the kit) for a Western.
This avoid me to do a 2nd transfection or duplicates just for a Western Blot.
Anyone has any ideia how can I concentrate this protein extract?
I'm trying acetone precipitation but I think I'm losing a lot of protein.
TCA precipitation is not a solution because it reacts with something in Passive Lysis Buffer.