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Protein Concentration from Passive Lysis Buffer

Protein Concentration from Passive Lysis Buffer - Protein Forum

Protein Concentration from Passive Lysis Buffer - Protein Forum


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Old 03-08-2010, 05:30 PM
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Default Protein Concentration from Passive Lysis Buffer



Hello everyone...

I doing some Luciferase assays (Promega).

But after reading the luciferase I want to recover all the protein extract (that is diluted on Passive Lysis Buffer from the kit) for a Western.
This avoid me to do a 2nd transfection or duplicates just for a Western Blot.

Anyone has any ideia how can I concentrate this protein extract?
I'm trying acetone precipitation but I think I'm losing a lot of protein.

TCA precipitation is not a solution because it reacts with something in Passive Lysis Buffer.

Ideas?
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