| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Protein Forum Protein Forum |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||
| |||
| Hi friends.. I am working with pET vector system. for expression of the enzme in E.coli BL21. I have to design primer with Nco1 site in forward primer. so can anybody will suggest me about the placing of ATG site of ORF...whether it should be in the primer's R.E site or else again i have to add in the primer aftre the R.E site. and one more thing for calculating the Tm and GC..in this primer designing. whethet i should consider the R.E site sequence along with the primer sequence..for the calculation or else i should exclude it... waiting for suggestion from scientific community |
| Tags |
| calculating , content , primer |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Bad primer design ? How can I know ? | surt | Bioinformatics | 4 | 10-07-2009 07:24 AM |
| Help in calculating Tm and GC content of primer | shrinivas Dengeti | Microbiology Forum | 0 | 09-12-2009 07:09 PM |
| Help in calculatiing Tm and GC content of primer | Shrinivas D | Protocols and Methods Forum | 0 | 09-12-2009 07:06 PM |
| Human Cytome Project - A framework for cytome exploration - Update 19 April 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 04-19-2005 07:16 AM |
| A framework for cytome exploration | Peter Van Osta | Cell Biology and Cell Culture | 0 | 03-14-2005 01:33 PM |