Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum


QUERY

QUERY - Protein Forum

QUERY - Protein Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-04-2009, 02:59 PM
Monica Mittal
Guest
 
Posts: n/a
Default QUERY



HI
I'M FACING PROBLEM IN PURIFYING A protein which is a dna binding protein but its showing a kind of hydrophobic behaviour as its max part is goinginto pellet after lysis.
moreover its not responding to ni2+ nta column also and the lysis sol. is not so clear that i go for gel filteration as itmay probably clog it
so can u suggest any solution?

monica



Love Cricket? Check out live scores, photos, video highlights and more. Click here [Only registered users see links. ]
Reply With Quote
  #2  
Old 09-08-2009, 01:58 PM
Dr Engelbert Buxbaum
Guest
 
Posts: n/a
Default QUERY

Am 04.09.2009, 10:59 Uhr, schrieb Monica Mittal <[Only registered users see links. ]>:


Does that mean you are expressing a poly-His tagged protein in bacteria?
If so, have you checked that your protein is not in inclusion bodies? How
did you lyse? How long and at what g did you try to clarify your lysate (1
h at 100000 g should do the trick)? Have you fragmented the bacterial DNA,
and are you sure that your protein is not binding to the fragments? Are
you protecting against oxidation and proteolysis?
Reply With Quote
Reply

Tags
query


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Pubreminer: a tool for PubMed query building and literature mining. webmaster Forum Biologie 0 06-11-2006 03:36 PM
Pubreminer: a tool for PubMed query building and literature mining. webmaster Protein Forum 0 06-11-2006 03:34 PM
Pubreminer: a tool for PubMed query building and literature mining. webmaster Cell Biology and Cell Culture 0 06-11-2006 03:33 PM
Pub re-miner: a tool for PubMed query building and literature mining. jkoster Forum Chemie 0 02-22-2006 09:26 PM
Pub re-miner: a tool for PubMed query building and literature mining. jkoster Cell Biology and Cell Culture 0 02-22-2006 09:23 PM


All times are GMT. The time now is 09:15 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11475 seconds with 16 queries