Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum

Invitation to Review: AJBR-08-041

Invitation to Review: AJBR-08-041 - Protein Forum

Invitation to Review: AJBR-08-041 - Protein Forum

LinkBack Thread Tools Display Modes
Old 06-08-2009, 03:50 PM
African Jourmal Biochemistry Research
Posts: n/a
Default Invitation to Review: AJBR-08-041

African Journal of Biochemistry Research

[Only registered users see links. ]

Dear Collegue,

We received a manuscript titled: **

The contribution of Ser463 residue to the enzymatic and autoprocessing
activities of *Escherichia coli *-glutamyltranspeptidase

Below you will find the abstract

A serine residue, Ser463, required for the proper function of *Escherichia
coli* g-glutamyltranspeptidase (*Ec*GGT) was identified by site-directed
mutagenesis on the basis of sequence alignment of human, pig, rat, and three
bacterial enzymes. Thr-, Asp-, and Lys-substituted variants were
overexpressed in *E. coli *M15 cells and the recombinant proteins were
purified to near homogeneity by nickel-chelate chromatography. With the
exception of S463T, the other two variants completely lost GGT activity,
implying the importance of this residue in *Ec*GGT. Moreover, substitution
of Ser463 with either Lys or Asp impaired the capability of autocatalytic
processing of the precursor into a- and b-subunit. Computer modeling showed
that the critical bonding distance of Gln390 C-Thr391 OG1 is significantly
increased in S463D and S463K, indicating that these distance changes might
be responsible for the lack of enzyme maturation. Measurements of intrinsic
tryptophan fluorescence revealed alteration of the microenvironment of
aromatic amino acid residues in S463D and S463K, while circular dichroism
spectra were nearly identical for wild-type and all mutant enzymes. The
temperature-dependent signal in the far-UV region for S463T was consistent
with that of wild-type enzyme but S463D and S463K showed a different
sensitivity towards temperature-induced denaturation. These results imply
that a significant conformational change occurred as a result of Asp- and

Key words: *Escherichia coli*, g-glutamyltranspeptidase, site-specific
mutagenesis, autocatalytic processing, tryptophan emission fluorescence,
circular dichroism

i am looking forward to your response if you are interested in reviewing.

Best regards,

Prof. Johnson Lin

Editor, African Journal of Biochemistry Research

E-mail: * **[Only registered users see links. ]* <[Only registered users see links. ]>

[Only registered users see links. ]
Reply With Quote

ajbr , invitation , review

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Invitation to Italy (and Sweden) vip/ba IPSI conference Protocols and Methods Forum 0 04-30-2004 02:03 PM
Invitation to Italy (and Sweden) vip/ba IPSI conference Cell Biology and Cell Culture 0 04-21-2004 10:48 AM
Pheromone Review Articles Art Ickles Chemistry Forum 1 12-14-2003 10:03 AM

All times are GMT. The time now is 04:51 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11749 seconds with 16 queries