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-   -   [Protein-analysis] sca-1 (http://www.molecularstation.com/forum/protein-forum/65210-%5Bprotein-analysis%5D-sca-1-a.html)

mmazan01 03-16-2009 09:37 PM

[Protein-analysis] sca-1
 

Hi - I am trying to detect Ly6 A/E (sca-1) from mouse lung on western blot.
I am using a monoclonal rat anti-mouse from ebiosciences. I have very clean
bands, no background, but at 75 kd instead of the expected 18 kd. Can
anyone shed light on this? Melissa
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Dr Engelbert Buxbaum 03-23-2009 12:10 PM

[Protein-analysis] sca-1
 
Am 16.03.2009, 17:37 Uhr, schrieb mmazan01 <[Only registered and activated users can see links. Click Here To Register...]>:


Sounds like you are looking at a tetramer. 18 kDa x 4 = 72 kDa, the
difference is within the expected error margin. Did you heat the sample
with DTT and SDS? What does the manufacturer of the antibody and the
literature say about what you should find?


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