Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum


Purification problem

Purification problem - Protein Forum

Purification problem - Protein Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-18-2009, 12:01 PM
Pipette Filler
Points: 54, Level: 1 Points: 54, Level: 1 Points: 54, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Purification problem



hello frnds


i m trying to purify protein in denatured condition, my protein is his tagged so i m using Ni-NTA resin and 8M urea to denature the protein. but most of the protein i am getting in my washing fractions, even single band i got in washing fraction at desired position.
when i m using elution buffer (ph 4.5) i m getting multiple bands with the desired protein.
Reply With Quote
Reply

Tags
problem , purification


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
fusion protein purification problem Chris McDermott-Roe Protocols and Methods Forum 2 08-05-2009 05:14 AM
Aggregation protein purification problem, help. xinnyu2002 Protocols and Methods Forum 0 07-15-2009 06:58 PM
His-tag protein purification under denaturing conditions Arabidop Molecular Biology Articles and Protocols 5 06-28-2009 06:05 AM
Does anybody have problem with system purchased from Marine Biotech? Kannika Siripattarapravat Zebrafish Forum 0 10-31-2006 10:08 PM
replies to indoor pest problem Dan Riggs Arabidopsis and Plant Biology 0 06-22-2004 04:59 PM


All times are GMT. The time now is 12:46 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11004 seconds with 16 queries