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#1
01-22-2009, 11:28 AM
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 Protein precipitation in HPLC buffer I would like to separate caseins using HPLC and it is known that acetonitrile precipitates proteins. Is it possible to use it Thank you very much  |
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#2
01-23-2009, 05:25 PM
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| Protein precipitation in HPLC buffer Am 22.01.2009, 08:28 Uhr, schrieb Mouldi SAIDI <[Only registered users see links. ].tn>: Sure. However, proteins - as opposed to small peptides - can not be separated by reverse phase chromatography (with a C18 column and acetonitrile gradient). You need other chromatographic principles. I have never worked with caseins, but consider ion exchange chromatography, chromatofocussing or hydrophobic interaction chromatography. A literature search on scholar.google.com may be a good first step. |
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| buffer , hplc , precipitation , protein |
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