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The fold of the protein is important, because not all cleavage sites are accessible to proteases. Conformation is important, too. There are several protease protection assays where binding of protein / activation / etc. can be monitored by increases or decreases in susceptibility to specific proteases.
Would any body help in letting us to know: how to identify scissile bonds in a protein sequence. Any web based tool or any triplet sequence related with Arg?
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